Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
Forelimb
MeSH Description
A front limb of a quadruped. (The Random House College Dictionary, 1980)

Attributes by original data submitter

Sample

source_name
forelimb
tissue
forelimb
age
Embryonic day 12.5&13.5
strain
C57BL/6
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and Smad4-DNA complexes were isolated with antibody. DNA sample was purified by Qiagen MinElute PCR Purification Kit, and was amplified with an Illumina ChIP-seq DNA Sample Prep Kit (IP-102-1001). DNA libraries were sent for sequencing with an Illumina HiSeq 2500 sequencer.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
119020230
Reads aligned (%)
96.9
Duplicates removed (%)
36.5
Number of peaks
663 (qval < 1E-05)

mm9

Number of total reads
119020230
Reads aligned (%)
96.5
Duplicates removed (%)
36.4
Number of peaks
877 (qval < 1E-05)

Base call quality data from DBCLS SRA