The hollow, muscular organ that maintains the circulation of the blood.
Attributes by original data submitter
Sample
source_name
heart, mdx
age
7 months
antibody
None
genotype/variation
IKK[beta]f/f
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated soluble chromatin was immunoprecipitated with 15 mg of H3K27ac antibody and pulled down by protein G sepharose beads equillibriated with yeast tRNA and BSA. Purified DNA (10 ng) was end-repaired and A-nucleotide overhangs were added by incubation with the Taq Klenow fragment lacking exonuclease activity. After the attachment of anchor sequences, fragments were PCR-amplified using Illumina-supplied primers. The purified DNA library products were evaluated using Bioanalyzer (Agilent).