Worms were frozen in liquid nitrogen prior to pulverization using a hammer as well as mortar and pestle. The resulting powder was fixed in 1,1 % formaldehyde. The sample was sonicated twice using a Bioruptor (Diagenode) for 15 times 30 sec ON, 30 sec OFF on high settings at 4°C. 5% of the supernatant was taken off as input and the protein concentration was determined using Bradford assay. The input was digested with 1 µl RNase A for 1 h at 37°C, the crosslink was reversed as described for the ChIP sample. 2-4 mg of extract was used for ChIPs. Library preparation was done following standard Bioo Scientific protocol and NEXTflex® qRNA-Seq™ Kit v2 (Bioo Scientific). Libraries were sequnced using single end sequencing length of a 75 nucleotides on a HiSeq400 machine (Illumina).