GSM3124999: T47D WT RNAP2 CDK9 ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CDK9
Cell type
Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
T47D cells
r5020 treatment
Untreated
chip antibody
CDK9
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
T47D-MTVL cells, without treatment or transfected with alpha-amanitin resistant HA-tagged wild type (WTr) versus R1810Ar mutant RNAP2 were cross-linked for 10 minutes with 1% formaldehyde. Lysate were sonicated to a DNA fragment size range of 200-300bp using a Biorupter sonicator (Diagenode). CDK9 Immunoprecipitated from , 150 μg chromatin with 15μg of anti-CDK9 (H-169.SC-8338)) antibody followed by 42μl of Protein A-Agarose Beads (Diagenode). Libraries were prepared using standadr Illumina instruction of NEBNext® ChIP-Seq Library Prep Reagent.