Antigen

Antigen Class

TFs and others

Antigen

CEBPA

Cell type

Cell type Class

Blood

Cell type

CFU-granulocytes

NA

NA

Sample

source_name

CFU-G

genotype

wildtype

cell markers

Lin- Sca1-cKit+ CD150- CD41- FcgRII/III(hi) CD115-

tissue

bone marrow

chip-antibody

14AA, Santa Cruz

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Genomic DNA extraction protocol not provided. Precipitated DNA were mixed with 0-2 ng E. Coli DNA, depending on the amount of ChIP DNA isolated. This mix was amplified using NEB Next ChIP-seq sample prep reagent set 1 (New England Biolabs) according to manufacturer's protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

55157649

Reads aligned (%)

77.8

Duplicates removed (%)

60.2

Number of peaks

5830 (qval < 1E-05)

mm9

Number of total reads

55157649

Reads aligned (%)

77.5

Duplicates removed (%)

60.2

Number of peaks

6132 (qval < 1E-05)