Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TCF7

Cell type

Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease

Attributes by original data submitter

Sample

source_name
TCF7-HA ChIPseq of MCF10DCIScells overexpressing TCF7
treatment
Dox
expression vector
TCF7
subtype
MCF10DCIS-TetTCF7
antibody
TCF7-HA
cell type
MCF10DCIS cells

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNeasy Mini Kit for RNA extraction, phenol-chloroform extraction for ChIP DNA Kapa mRNA Hyper Pepe Kit, Rubicon ThruPLEX DNA-seq Kit

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
28889268
Reads aligned (%)
48.6
Duplicates removed (%)
46.5
Number of peaks
43196 (qval < 1E-05)

hg38

Number of total reads
28889268
Reads aligned (%)
58.0
Duplicates removed (%)
38.2
Number of peaks
43994 (qval < 1E-05)

Base call quality data from DBCLS SRA