Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BACH1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Bach1 knockout_hESC
cell type
embryonic stem cell
genotype/variation
Bach1 knockout
passages
30-60
chip anitbody
H3K27me3 (millipore, cat##2699642)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was harvested using Trizol reagent. TrueLib mRNA Library building kit (ExCell Bio , Cat # NGS00-2012) was used with 1 ug of total RNA for the construction of sequencing libraries. Immunoprecipitated DNA was harvested from Samples which were incubated with 3–5 μg of antibody during CHIP assay. KAPA Hyper Prep Kits with PCR Library Amplification/Illumina series( KAPA biosystems, cat# KK8500) was used with 10-100ng DNA for the construction of sequencing libraries. RNA-seq and CHIP-seq libraries were prepared for sequencing using standard protocols In accordance with the instructions.

Sequencing Platform

instrument_model
HiSeq X Ten

hg19

Number of total reads
34943864
Reads aligned (%)
32.2
Duplicates removed (%)
14.9
Number of peaks
359 (qval < 1E-05)

hg38

Number of total reads
34943864
Reads aligned (%)
32.7
Duplicates removed (%)
14.6
Number of peaks
431 (qval < 1E-05)

Base call quality data from DBCLS SRA