RNA was harvested using Trizol reagent. TrueLib mRNA Library building kit (ExCell Bio , Cat # NGS00-2012) was used with 1 ug of total RNA for the construction of sequencing libraries. Immunoprecipitated DNA was harvested from Samples which were incubated with 3–5 μg of antibody during CHIP assay. KAPA Hyper Prep Kits with PCR Library Amplification/Illumina series( KAPA biosystems, cat# KK8500) was used with 10-100ng DNA for the construction of sequencing libraries. RNA-seq and CHIP-seq libraries were prepared for sequencing using standard protocols In accordance with the instructions.