Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ERG

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived cardiac cells
NA
NA

Attributes by original data submitter

Sample

source_name
ERRgamma KO hiPSC-CMs
genotype
ERRgamma KO
cell type
hiPSC-derived cardioyocytes
day
D22
chip antibody
ERRg (generated with collaborator)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and ERRg-DNA complexes were isolated with the antibody. Libraries were prepared according to NuGen The Ovation® Ultralow Library System V2 (Part# 0801-0303) with 2 mg DNA sample.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
27241879
Reads aligned (%)
73.1
Duplicates removed (%)
24.6
Number of peaks
2251 (qval < 1E-05)

hg38

Number of total reads
27241879
Reads aligned (%)
73.8
Duplicates removed (%)
24.2
Number of peaks
2249 (qval < 1E-05)

Base call quality data from DBCLS SRA