GSM3110062: T47D R5020 PR-1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
PGR
Cell type
Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
T47D_R5020_PR
cell line
T47D
treatment
R5020
chip antibody
PR (Santa Cruz SC-7208X)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was extracted using SimpleChIP chromatin IP kit (cell signaling) and immunoprecipitated with PR antibody. Libraries were prepared using Kapa Biosystems Hyper Prep kit (#KK8500) according to manufacturer's protocol. 1ng of DNA was used and 12 cycle library amplification was performed.