Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PGR

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D_R5020_PR
cell line
T47D
treatment
R5020
chip antibody
PR (Santa Cruz SC-7208X)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was extracted using SimpleChIP chromatin IP kit (cell signaling) and immunoprecipitated with PR antibody. Libraries were prepared using Kapa Biosystems Hyper Prep kit (#KK8500) according to manufacturer's protocol. 1ng of DNA was used and 12 cycle library amplification was performed.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
22116014
Reads aligned (%)
82.6
Duplicates removed (%)
15.1
Number of peaks
30398 (qval < 1E-05)

hg38

Number of total reads
22116014
Reads aligned (%)
83.8
Duplicates removed (%)
14.4
Number of peaks
30512 (qval < 1E-05)

Base call quality data from DBCLS SRA