Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D_VC_input
cell line
T47D
treatment
VC
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was extracted using SimpleChIP chromatin IP kit (cell signaling) and immunoprecipitated with PR antibody. Libraries were prepared using Kapa Biosystems Hyper Prep kit (#KK8500) according to manufacturer's protocol. 1ng of DNA was used and 12 cycle library amplification was performed.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
28638327
Reads aligned (%)
98.7
Duplicates removed (%)
35.6
Number of peaks
603 (qval < 1E-05)

hg19

Number of total reads
28638327
Reads aligned (%)
97.8
Duplicates removed (%)
37.0
Number of peaks
565 (qval < 1E-05)

Base call quality data from DBCLS SRA