GSM3109178: H3K27Ac ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Blood
Cell type
T-ALL
NA
NA
Attributes by original data submitter
Sample
source_name
Human T-ALL PDX
host strain
NOG mice (3-4 week-old).
host gender
female
engrafted with
MNCs isolated from the patient BM sample
sample type
T-ALL Patient Dirived Xenograft (PDX) sample
chip antibody
H3K27Ac (ab4729, Abcam)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked with 1% formaldehyde for 10 min at room temperature and formaldehyde was then inactivated by the addition of 125 mM glycine for 5min. Sonicated DNA fragments with 100–300 bp were pre-cleared and then immunoprecipitated with Protein A+G Magnetic beads coupled with Anti-H3K27Ac antibody (ab4729, Abcam). After reverse crosslinking, immunoprecipitated DNAs and input DNAs were end-repaired and ligated adapters to the DNA fragments using NEBNext Ultra End-Repair/dA-Tailing Module (E7442, NEB) and NEBNext Ultra Ligation Module (E7445, NEB).