Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ELF1

Cell type

Cell type Class
Prostate
Cell type
RWPE-1
Primary Tissue
Prostate
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
immortalized normal prostate cells
cell line
RWPE-1
chip antibody
ELF1 (sc-631, Santa Cruz Biotechnology)
growth protocol
RWPE1 cells were grown in Keratinocyte SFM media supplemented with bovine pitutiary extract, human recombinant epidermal growth factor, and penicillin/streptomycin

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP DNA was isolated as described in Hollenhorst et. al., Genes and Development, 2011 Sequencing libraries were created as described in Plotnik et.al. Nucleic Acids Research. 2014.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
73947047
Reads aligned (%)
84.7
Duplicates removed (%)
55.3
Number of peaks
1349 (qval < 1E-05)

hg19

Number of total reads
73947047
Reads aligned (%)
84.1
Duplicates removed (%)
56.8
Number of peaks
1331 (qval < 1E-05)

Base call quality data from DBCLS SRA