GSM3105172: ChIP-Bcl6, from inguinal adipose; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Adipocyte
Cell type
Inguinal adipose
NA
NA
Attributes by original data submitter
Sample
source_name
inguinal adipose
strain background
C57BL/6
genotype/variation
Adipoq-Cre;Sun1-sfGFP-myc
tissue
inguinal adipose
primary antibody
guinea pig anti mouse Bcl6, polyconal (custom)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Inguinal and perigonadal adipose was dissected and finely minced. Tissue was subjected to cross linking in disuccinimidyl glutarate for 30 minutes followed by 1% formaldehyde for 10 minutes. Reaction was quenched with glycine. Tissue was dounced to release nuclei. Sun1 positive nuclei from adipocytes were collected by addition of c-myc magnetic beads. Enriched nuclei were sonicated to shear the chromatin and subjected to immunoprecipitation. Precipitates were washed and DNA was purified by column (Qiagen Min-Elute). KAPA Hyper Prep Kit (Kapa Biosciences)