ChIP was performed as described in CM Weber, JG Henikoff & S Henikoff, H2A.Z nucleosomes enriched over active genes are homotypic, Nat Struct Mol Bio 2010 Dec;17(12):1500-7, PMID 21057526 and SS Teves & S Henikoff Heat shock reduces stalled RNA polymerase II and nucleosome turnover genome-wide. Genes Dev. 2011 Nov 15;25(22):2387-97. PubMed PMID: 22085965 A modified Solexa Illumina library construction protocol was used as previously described in SS Teves & S Henikoff Heat shock reduces stalled RNA polymerase II and nucleosome turnover genome-wide. Genes Dev. 2011 Nov 15;25(22):2387-97. PubMed PMID: 22085965