Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Trl

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
GAF n-ChIP S2
cell line
S2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as described in CM Weber, JG Henikoff & S Henikoff, H2A.Z nucleosomes enriched over active genes are homotypic, Nat Struct Mol Bio 2010 Dec;17(12):1500-7, PMID 21057526 and SS Teves & S Henikoff Heat shock reduces stalled RNA polymerase II and nucleosome turnover genome-wide. Genes Dev. 2011 Nov 15;25(22):2387-97. PubMed PMID: 22085965 A modified Solexa Illumina library construction protocol was used as previously described in SS Teves & S Henikoff Heat shock reduces stalled RNA polymerase II and nucleosome turnover genome-wide. Genes Dev. 2011 Nov 15;25(22):2387-97. PubMed PMID: 22085965

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
33235249
Reads aligned (%)
84.2
Duplicates removed (%)
14.0
Number of peaks
3450 (qval < 1E-05)

dm6

Number of total reads
33235249
Reads aligned (%)
83.8
Duplicates removed (%)
16.6
Number of peaks
4597 (qval < 1E-05)

Base call quality data from DBCLS SRA