Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Epitope tags

Cell type

Cell type Class
Digestive tract
Cell type
DLD-1
Primary Tissue
Colon
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
DLD1 colon cancer cells
cell line
DLD1 colon cancer cells
genotype/variation
dnTCF1Emut
chip antibody
Anti-FLAG Beads Sigma (A2220)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Used anti-FLAG conjugated magnetic beads from Sigma (A2220) to immunoprecipitate FLAG-tagged dnTCF1EWT and FLAG-tagged dnTCF1Emut All libraries were prepared using the NEXTflex ChIP Seq kit from Biooscientific using 10ng as starting material

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
48280391
Reads aligned (%)
96.9
Duplicates removed (%)
17.1
Number of peaks
1451 (qval < 1E-05)

hg19

Number of total reads
48280391
Reads aligned (%)
96.1
Duplicates removed (%)
17.8
Number of peaks
1088 (qval < 1E-05)

Base call quality data from DBCLS SRA