Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Neural
Cell type
T98G
Primary Tissue
Brain
Tissue Diagnosis
Glioblastoma

Attributes by original data submitter

Sample

source_name
T98G
cell line
T98G
cell type
Glioblastoma multiforme cell line
chip antibody
H3K4me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Antibodies used were H3K4me3 (EMD Millipore, 07-473) and H3K27me3 (Millipore 07-449). Libraries were prepared according to the New England Biolabs NEBNext library prep kit (E6240L). Ampure XP beads (A63881) were used for size-selection and clean-up steps.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
19893921
Reads aligned (%)
94.8
Duplicates removed (%)
4.1
Number of peaks
24130 (qval < 1E-05)

hg19

Number of total reads
19893921
Reads aligned (%)
94.7
Duplicates removed (%)
4.1
Number of peaks
24013 (qval < 1E-05)

Base call quality data from DBCLS SRA