Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Others
Cell type
Fibroblasts
NA
NA

Attributes by original data submitter

Sample

source_name
diploid fibroblast
factor
p53
condition
Growing
antibody
anti p53 mouse monoclonal (DO-1) Sigma

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 4 sequencing on an Illumina Genome Analyzer according to the manufacturer’s recommendationas described in Chandra, T. et al. Independence of Repressive Histone Marks and ChromatinCompaction during Senescent Heterochromatic Layer Formation. Mol Cell 47, 203–214 (2012)

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
63142812
Reads aligned (%)
86.3
Duplicates removed (%)
11.2
Number of peaks
1797 (qval < 1E-05)

hg38

Number of total reads
63142812
Reads aligned (%)
88.6
Duplicates removed (%)
9.7
Number of peaks
1999 (qval < 1E-05)

Base call quality data from DBCLS SRA