Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Cell line

Cell type

S2R+

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

Input_RnpS1KD

cell line

S2R+ Cells

cell type

Embryonic

chip antibody

Input control

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was recovered after reverse crosslinking and phenol chloroform extraction. After precipitating and pelleting, DNA was dissolved in 30 _l of TE After checking enrichment the recovered DNA was converted into libraries using NebNext Ultra DNA II library preparation kit, following manufacturer's protocol

Sequencing Platform

instrument_model

NextSeq 500

dm6

Number of total reads

15146852

Reads aligned (%)

78.9

Duplicates removed (%)

10.3

Number of peaks

2301 (qval < 1E-05)

dm3

Number of total reads

15146852

Reads aligned (%)

80.0

Duplicates removed (%)

9.5

Number of peaks

2987 (qval < 1E-05)