Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryonic fibroblast
Cell type
NIH/3T3
Primary Tissue
Embryo
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
NIH3T3_input
cell line
NIH3T3
cell type
Mouse embryo fibroblast
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the Illumina TruSeq Nano DNA Library Prep Kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
49734501
Reads aligned (%)
97.6
Duplicates removed (%)
15.6
Number of peaks
533 (qval < 1E-05)

mm9

Number of total reads
49734501
Reads aligned (%)
97.3
Duplicates removed (%)
15.5
Number of peaks
616 (qval < 1E-05)

Base call quality data from DBCLS SRA