Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Adult
Cell type
Adult head
NA
NA

Attributes by original data submitter

Sample

source_name
fly head
tissue
head
age
3 days
genotype
Pcl,Su(z)12 double mutant
chip antibody
N/A

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, 5% mouse epigenome was used as internal control for H3K27me3 IP. ChIP libraries were prepared according to instructions of NEB DNA library prep kit (NEB, E7370L). RNA libraries were prepared according to instructions of NEB Ultra RNA library prep kit (NEB, E7530L).

Sequencing Platform

instrument_model
Illumina MiSeq

dm6

Number of total reads
3384442
Reads aligned (%)
76.5
Duplicates removed (%)
6.5
Number of peaks
1056 (qval < 1E-05)

dm3

Number of total reads
3384442
Reads aligned (%)
77.2
Duplicates removed (%)
4.6
Number of peaks
644 (qval < 1E-05)

Base call quality data from DBCLS SRA