Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TAL1

Cell type

Cell type Class
Cardiovascular
Cell type
Primary endothelial cells
NA
NA

Attributes by original data submitter

Sample

source_name
Human primary endothelial colony-forming cells
source
ECFCs derived from human umbilical cord blood
antibody
anti-TAL1 (C-21, Santa-Cruz)
treatment
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Binding of TAL1 transcription factor was measured using a ChIP protocol as previously described (Palii, 2011). Chromatin from 0.5x10^8 cells was fragmented to a size range of 100-300 bases with a Bioruptor (Diagenode) at 4°C. Solubilized chromatin was immunoprecipitated with an antibody against TAL1 (C-21, Santa-Cruz) or an isotype IgG control. Antibody-chromatin complexes were pulled-down using Dynabeads-Protein G, washed and eluted. After cross-link reversal and proteinase K treatment, immunoprecipitated DNA was extracted with phenol-chloroform. ChIPed DNA was amplified using the Illumina protocol with modifications described in (Palii, 2011).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
79835196
Reads aligned (%)
45.9
Duplicates removed (%)
47.4
Number of peaks
51800 (qval < 1E-05)

hg38

Number of total reads
79835196
Reads aligned (%)
47.2
Duplicates removed (%)
46.3
Number of peaks
51998 (qval < 1E-05)

Base call quality data from DBCLS SRA