Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GRHL2

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
WS8
cell line
WS8
treatment
VEH
treatment time
45min
factor
GRHL2
chip antibody
Sigma, HPA004820
replicate
Rep1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell pellets were thawed on ice, and then resuspended in Lysis Buffer containing 1% SDS, 0.25mM EDTA, 2.5mM Tris-HCl, pH 8.0, protease inhibitor cocktail. Cell lysates were sonicated using the Covaris. KAPA HTP

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
30124013
Reads aligned (%)
83.4
Duplicates removed (%)
9.9
Number of peaks
15781 (qval < 1E-05)

hg38

Number of total reads
30124013
Reads aligned (%)
85.4
Duplicates removed (%)
8.6
Number of peaks
16036 (qval < 1E-05)

Base call quality data from DBCLS SRA