Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GRHL2

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
TAMR
cell line
TAMR
treatment
VEH
treatment time
45min
factor
GRHL2
chip antibody
Sigma, HPA004820
replicate
Rep3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell pellets were thawed on ice, and then resuspended in Lysis Buffer containing 1% SDS, 0.25mM EDTA, 2.5mM Tris-HCl, pH 8.0, protease inhibitor cocktail. Cell lysates were sonicated using the Covaris. KAPA HTP

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
34726257
Reads aligned (%)
81.0
Duplicates removed (%)
13.3
Number of peaks
35942 (qval < 1E-05)

hg38

Number of total reads
34726257
Reads aligned (%)
82.8
Duplicates removed (%)
12.1
Number of peaks
36260 (qval < 1E-05)

Base call quality data from DBCLS SRA