Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
REST

Cell type

Cell type Class
Blood
Cell type
GM12878
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-Barr Virus

Attributes by original data submitter

Sample

source_name
GM12878-NRSF
biomaterial_provider
GM12878
cell line
GM12878
chip antibody
mouse monoclonal NRSF
chip antibody info
custom generated (Johnson et al. 2007, PMID: 17540862)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed using 1% formaldehyde (NRSF samples) or 1% formaldehyde at 37C (p300 samples). Libraries were generated following Illumina's DNA Sample Prep Kit protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
21186910
Reads aligned (%)
56.3
Duplicates removed (%)
15.5
Number of peaks
4688 (qval < 1E-05)

hg38

Number of total reads
21186910
Reads aligned (%)
57.6
Duplicates removed (%)
14.2
Number of peaks
4407 (qval < 1E-05)

Base call quality data from DBCLS SRA