Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Bhlhe40

Cell type

Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
In vitro TH1-polarized CD4+ T cells
cell type
CD4+ T cell
bhlhe40
KO (Bhlhe40-/-)
strain
C57BL/6
chip antibody
anti-Bhlhe40 (Novus Biologicals NB100-1800, Lot C1)
treatment
50ng/mL PMA, 1uM Ionomycin for 1.5 hours

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Stimulated cells were fixed for 10 minutes at room temperature with 1% paraformaldehyde with shaking. Cross-linked chromatin was fragmented by sonication and then immunoprecipitated with polyclonal rabbit anti-Bhlhe40 antibody (Novus Biologicals NB100-1800, Lot A or Lot C1). After immunoprecipitation, DNA was purified by the GenElute PCR clean-up kit (Sigma). Standard ChIP library prep for Illumina sequencing

Sequencing Platform

instrument_model
Illumina HiSeq 3000

mm10

Number of total reads
37222376
Reads aligned (%)
94.5
Duplicates removed (%)
79.7
Number of peaks
1363 (qval < 1E-05)

mm9

Number of total reads
37222376
Reads aligned (%)
94.4
Duplicates removed (%)
79.8
Number of peaks
1393 (qval < 1E-05)

Base call quality data from DBCLS SRA