anti-Bhlhe40 (Novus Biologicals NB100-1800, Lot C1)
treatment
50ng/mL PMA, 1uM Ionomycin for 1.5 hours
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Stimulated cells were fixed for 10 minutes at room temperature with 1% paraformaldehyde with shaking. Cross-linked chromatin was fragmented by sonication and then immunoprecipitated with polyclonal rabbit anti-Bhlhe40 antibody (Novus Biologicals NB100-1800, Lot A or Lot C1). After immunoprecipitation, DNA was purified by the GenElute PCR clean-up kit (Sigma). Standard ChIP library prep for Illumina sequencing