Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Spi1

Cell type

Cell type Class
Blood
Cell type
HAFTL
NA
NA

Attributes by original data submitter

Sample

source_name
Haftl pre-B cells
cell type
haftl derived C10 cells
time after induction
3 hours
chip antibody
PU.1 (sc-352, Santa Cruz)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed as described previously (van Oevelen et al, 2008, PMID:20956564). DNA libraries of C/EBPa and C/EBPb enriched chromatin fragments were prepared using Illumina's reagents and instructions. All other libraries were prepared according to manufacturer’s instructions with some modifications (Asp et al, 2011, PMID: 21551099). All libraries were sequenced on the Illumina 2AAIIx sequencer except C/EBPa and PU.1 libraries generated 10, 30 and 60 minutes post induction and pax5 (0 hours) and Foxo1 (0 hours) that were sequenced using the Illumina Hiseq2000 sequencer.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
17675460
Reads aligned (%)
70.0
Duplicates removed (%)
17.1
Number of peaks
20368 (qval < 1E-05)

mm9

Number of total reads
17675460
Reads aligned (%)
69.8
Duplicates removed (%)
17.2
Number of peaks
20402 (qval < 1E-05)

Base call quality data from DBCLS SRA