Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ONECUT2

Cell type

Cell type Class
Digestive tract
Cell type
MKN-74
Primary Tissue
Stomach
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MKN74
cell line
MKN74
cell type
gastric cancer cell line
genotype/variation
ONECUT2 overexpression
chip antibody
anti-ONECUT2 (proteintech, catalog no. 21916-1-AP)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The RNA sequencing library was prepared using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA). and For ChIP-seq, Cells were fixed using 1% formaldehyde, lysed and sonicated using a Bioruptor (Diagenode Inc.). RNA libraries were prepared for sequencing using standard Illumina protocols. For ChIP-seq, immunoprecipitation was performed with anti-ONECUT2(proteintech, catalog no. 21916-1-AP). 250-400 bp genomic libraries were generated from the input and immunoprecipitated DNA and sequenced using Illumina NextSeq_500 to generate 75 bp single-end reads.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
53864048
Reads aligned (%)
68.4
Duplicates removed (%)
20.7
Number of peaks
126416 (qval < 1E-05)

hg38

Number of total reads
53864048
Reads aligned (%)
69.1
Duplicates removed (%)
20.2
Number of peaks
126743 (qval < 1E-05)

Base call quality data from DBCLS SRA