Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ONECUT2

Cell type

Cell type Class
Digestive tract
Cell type
AGS
Primary Tissue
Stomach
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
AGS
cell line
AGS
cell type
gastric cancer cell line
genotype/variation
ONECUT2 overexpression
chip antibody
anti-ONECUT2 (proteintech, catalog no. 21916-1-AP)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The RNA sequencing library was prepared using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA). and For ChIP-seq, Cells were fixed using 1% formaldehyde, lysed and sonicated using a Bioruptor (Diagenode Inc.). RNA libraries were prepared for sequencing using standard Illumina protocols. For ChIP-seq, immunoprecipitation was performed with anti-ONECUT2(proteintech, catalog no. 21916-1-AP). 250-400 bp genomic libraries were generated from the input and immunoprecipitated DNA and sequenced using Illumina NextSeq_500 to generate 75 bp single-end reads.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
52359000
Reads aligned (%)
83.2
Duplicates removed (%)
12.0
Number of peaks
10865 (qval < 1E-05)

hg38

Number of total reads
52359000
Reads aligned (%)
84.8
Duplicates removed (%)
11.0
Number of peaks
10985 (qval < 1E-05)

Base call quality data from DBCLS SRA