GSM3094436: AGS control input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Digestive tract
Cell type
AGS
Primary Tissue
Stomach
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
AGS
cell line
AGS
cell type
gastric cancer cell line
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The RNA sequencing library was prepared using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA). and For ChIP-seq, Cells were fixed using 1% formaldehyde, lysed and sonicated using a Bioruptor (Diagenode Inc.). RNA libraries were prepared for sequencing using standard Illumina protocols. For ChIP-seq, immunoprecipitation was performed with anti-ONECUT2(proteintech, catalog no. 21916-1-AP). 250-400 bp genomic libraries were generated from the input and immunoprecipitated DNA and sequenced using Illumina NextSeq_500 to generate 75 bp single-end reads.