Antigen

Antigen Class

TFs and others

Antigen

BRD9

Cell type

Cell type Class

Blood

Cell type

Jurkat

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Lymphocytic

Sample

source_name

JURKAT (T-ALL)

cell type

T-ALL

cell line

JURKAT

chip antibody

BRD9

antibody manufacturer

Abcam

antibody catalog number

137245

antibody lot number

GR257571-3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed using standard protocols (Millipore, Billerica, MA). Specifically, cells were fixed in 1% formaldehyde (Sigma Aldrich, F8775) for 10min at 37C and quenched with 125mM glycine for 5min at 37C. After washing, nuclei were sonicated using Covaris Sonicator, and the supernatant was used for immunoprecipitation with the indicated antibody. ChIP-sequencing libraries were prepared with the Rubicon Thruplex DNAseq prep kit using standard protocols.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

24082851

Reads aligned (%)

95.2

Duplicates removed (%)

20.1

Number of peaks

3401 (qval < 1E-05)

hg19

Number of total reads

24082851

Reads aligned (%)

92.5

Duplicates removed (%)

25.5

Number of peaks

2427 (qval < 1E-05)