Antigen

Antigen Class

TFs and others

Antigen

SS18

Cell type

Cell type Class

Muscle

Cell type

SYO-1

NA

NA

Sample

source_name

SYO1 (Synovial Sarcoma)

cell type

Synovial Sarcoma

cell line

SYO1

chip antibody

SS18

antibody manufacturer

CST

antibody catalog number

21792s

antibody lot number

Lot 1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed using standard protocols (Millipore, Billerica, MA). Specifically, cells were fixed in 1% formaldehyde (Sigma Aldrich, F8775) for 10min at 37C and quenched with 125mM glycine for 5min at 37C. After washing, nuclei were sonicated using Covaris Sonicator, and the supernatant was used for immunoprecipitation with the indicated antibody. ChIP-sequencing libraries were prepared with the Rubicon Thruplex DNAseq prep kit using standard protocols.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

47256134

Reads aligned (%)

97.7

Duplicates removed (%)

18.7

Number of peaks

60566 (qval < 1E-05)

hg19

Number of total reads

47256134

Reads aligned (%)

97.3

Duplicates removed (%)

19.3

Number of peaks

60405 (qval < 1E-05)