Antigen

Antigen Class

TFs and others

Antigen

SMARCA4

Cell type

Cell type Class

Blood

Cell type

MOLM-13

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

MOLM13 (AML)

cell type

AML

cell line

MOLM13

chip antibody

SMARCA4

antibody manufacturer

Abcam

antibody catalog number

EPNCIR111A

antibody lot number

GR150844-12

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed using standard protocols (Millipore, Billerica, MA). Specifically, cells were fixed in 1% formaldehyde (Sigma Aldrich, F8775) for 10min at 37C and quenched with 125mM glycine for 5min at 37C. After washing, nuclei were sonicated using Covaris Sonicator, and the supernatant was used for immunoprecipitation with the indicated antibody. ChIP-sequencing libraries were prepared with the Rubicon Thruplex DNAseq prep kit using standard protocols.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

57070615

Reads aligned (%)

96.6

Duplicates removed (%)

33.1

Number of peaks

75805 (qval < 1E-05)

hg19

Number of total reads

57070615

Reads aligned (%)

96.2

Duplicates removed (%)

33.6

Number of peaks

75383 (qval < 1E-05)