GSM1290021: ChIP-4b mutant-input; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Others
Cell type
T3M-1 Cl-10
Primary Tissue
Oral Cavity
Tissue Diagnosis
Carcinoma Squamous Cell
Attributes by original data submitter
Sample
source_name
Oral cancer cell line
cell
T3M-1 Cl-10
genotype
L211M
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
T3M-1 Cl-10 cells were infected with a mock retrovirus or virus generated from pMXS-ires-EGFP expressing FLAG-epitope–tagged ELF4 or ELF4(L211M). ChIP was conducted with anti-FLAG M2 antibody (Sigma-Aldrich). The precipitated DNA fragments were subjected to massively parallel sequencing with Genome Analyzer IIx (Illumina) for 51 bases from both ends of the fragments. ChIP-seq libraries were prepared using Illumina NEBNext ChIP-Seq Library Prep Reagent Set following the manufacturer's protocols.