Curated Sample Data


Genome
ce10
Antigen Class
TFs and others
Antigen
GFP
Cell type Class
Adult
Cell type
Adult

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
Whole animals
strain
DLS396
developmental stage
Day 1 adults
genotype
ceh-60(rhd116); gaIs285
chip antibody
anti-GFP (3E6, Thermo Fisher, A-11120)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Animals were harvested in M9 buffer, washed, and ground to a find powder with a mortar and pestle in liquid Nitrogen. Whole animal lysates were crosslinked with 2% formaldehyde for 30 minutes, and the genomic DNA was sheared using a Bioruptor Plus (Diagenode). The resulting chromatin was immunoprecipitated with anti-HA or anti-GFP antibodies. ChIP-Seq libraries were prepared for sequencing using the KAPA Hyper Prep Kit (Kapa Biosystems) according to the manufacturer's instructions.

Platform Information


instrument_model
Illumina HiSeq 4000

External Database Query

Logs in read processing pipeline


Number of total reads
71485065
Reads aligned (%)
85.9
Duplicates removed (%)
92.3
Number of peaks
3265 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA