Curated Sample Data


Genome
ce10
Antigen Class
TFs and others
Antigen
Epitope tags
Cell type Class
Adult
Cell type
Adult

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
Whole animals
strain
DLS395
developmental stage
Day 1 adults
genotype
ceh-60(rhd116)
chip antibody
anti-HA (mIgG1, Pierce, 26181)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Animals were harvested in M9 buffer, washed, and ground to a find powder with a mortar and pestle in liquid Nitrogen. Whole animal lysates were crosslinked with 2% formaldehyde for 30 minutes, and the genomic DNA was sheared using a Bioruptor Plus (Diagenode). The resulting chromatin was immunoprecipitated with anti-HA or anti-GFP antibodies. ChIP-Seq libraries were prepared for sequencing using the KAPA Hyper Prep Kit (Kapa Biosystems) according to the manufacturer's instructions.

Platform Information


instrument_model
Illumina HiSeq 4000

External Database Query

Logs in read processing pipeline


Number of total reads
70025667
Reads aligned (%)
73.1
Duplicates removed (%)
89.3
Number of peaks
4316 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA