Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Epitope tags

Cell type

Cell type Class
Adult
Cell type
Adult
NA
NA

Attributes by original data submitter

Sample

source_name
Whole animals
strain
DLS395
developmental stage
Day 1 adults
genotype
ceh-60(rhd116)
chip antibody
anti-HA (3F10, Sigma-Aldrich, 11867423001)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Animals were harvested in M9 buffer, washed, and ground to a find powder with a mortar and pestle in liquid Nitrogen. Whole animal lysates were crosslinked with 2% formaldehyde for 30 minutes, and the genomic DNA was sheared using a Bioruptor Plus (Diagenode). The resulting chromatin was immunoprecipitated with anti-HA or anti-GFP antibodies. ChIP-Seq libraries were prepared for sequencing using the KAPA Hyper Prep Kit (Kapa Biosystems) according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 4000

ce11

Number of total reads
64808971
Reads aligned (%)
95.5
Duplicates removed (%)
86.2
Number of peaks
7861 (qval < 1E-05)

ce10

Number of total reads
64808971
Reads aligned (%)
95.5
Duplicates removed (%)
86.2
Number of peaks
7863 (qval < 1E-05)

Base call quality data from DBCLS SRA