Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
DDX5

Cell type

Cell type Class
Breast
Cell type
BT-549
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
BT549 cells
cell line
BT549
cell type
ERalpha-negtive breast cancer cells
chip antibody
DDX5 (ab21696, Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei, IgG, Fra-1 and DDX5 complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
42792776
Reads aligned (%)
84.0
Duplicates removed (%)
13.8
Number of peaks
1577 (qval < 1E-05)

hg38

Number of total reads
42792776
Reads aligned (%)
86.6
Duplicates removed (%)
12.0
Number of peaks
1534 (qval < 1E-05)

Base call quality data from DBCLS SRA