Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Hematopoietic Stem Cells
MeSH Description
Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Attributes by original data submitter

Sample

source_name
Mouse HPCs
tissue
Hematopoietic System
antibody
IgG

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fix 1% paraformaldehyde, nuclear purification with 0.5% NP40, DNA solubilization and sonication in 1% SDS followed by Co-IP with antibody of interest Illumina Truseq LT

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
18705559
Reads aligned (%)
2.8
Duplicates removed (%)
69.0
Number of peaks
21 (qval < 1E-05)

mm9

Number of total reads
18705559
Reads aligned (%)
2.8
Duplicates removed (%)
69.6
Number of peaks
20 (qval < 1E-05)

Base call quality data from DBCLS SRA