Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Irf8

Cell type

Cell type Class
Blood
Cell type
Dendritic Cells
MeSH Description
ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Attributes by original data submitter

Sample

source_name
Flt3 ligand derived dendritic cells
genotype
Irf8+/+ Batf3+/+
strain
C57BL/6
chip antibody
ICSBP Antibody (Santa Cruz biotechnology inc., C-19)
cell type
DC

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and IRF8-DNA complexes were isolated with antibody. ChIP DNA was blunt ended, had addition of “A” base to 3’ end, and had sequencing adapters ligated to the ends. The fragments were size selected to 200-600 base pairs, and underwent amplification for 15 cycles.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
25008376
Reads aligned (%)
98.0
Duplicates removed (%)
19.8
Number of peaks
27194 (qval < 1E-05)

mm9

Number of total reads
25008376
Reads aligned (%)
97.8
Duplicates removed (%)
19.9
Number of peaks
28132 (qval < 1E-05)

Base call quality data from DBCLS SRA