Antigen

Antigen Class

TFs and others

Antigen

Junb

Cell type

Cell type Class

Blood

Cell type

Dendritic Cells

MeSH Description

ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Sample

source_name

BMDCs, LPS, 6 hrs, JunB ChIP

strain/background

C57BL/6

cell type

bone marrow-derived dendritic cells

treatment

LPS

timepoint

6 hrs

chip antibody

anti-JunB (Santa Cruz Biotechnology, sc-73x (210x), Lot# D1210)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

20000000 cells were harvested, crosslinked with formaldehyde, sonicated, IPed with appropriate antibodies and reverse-crosslinked to obtain 300-500 bp DNA fragments. These DNA fragments were ligated to NuGen Ovation multiplexing adapters for sequencing on the Illumina HiSeq 2000.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

19057068

Reads aligned (%)

96.8

Duplicates removed (%)

34.7

Number of peaks

336 (qval < 1E-05)

mm9

Number of total reads

19057068

Reads aligned (%)

96.6

Duplicates removed (%)

34.6

Number of peaks

312 (qval < 1E-05)