Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic stem cells
cell type
V6.5
chip antibody
None
antibody catalog
N/A
young_id
20170928_6368

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Purified immunoprecipitated DNA was prepared for sequencing using the Swift Biosciences ChIP kit by the Whitehead Institute Genome Technology Core. Whole cell extracts were sonicated using a Covaris S220 under manufacturer's recommended settings for 12 minutes to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using indicated antibodies.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
55077104
Reads aligned (%)
96.6
Duplicates removed (%)
11.4
Number of peaks
583 (qval < 1E-05)

mm9

Number of total reads
55077104
Reads aligned (%)
96.4
Duplicates removed (%)
11.4
Number of peaks
678 (qval < 1E-05)

Base call quality data from DBCLS SRA