Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Unclassified

Cell type

Unclassified

NA

NA

Sample

source_name

Cell culture

growth protocol

grown in RPMI supplemented with 10% FBS, penicillin and streptomycin.

experiment

ChIP-H3K4me3

antibodies

H3K4me3 (Abcam, ab1012)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Native ChIP was done as previously described (Hasson D., et al., The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013 Jun;20(6):687-95). Libraries for ChIP-seq were done as previously described (Hasson D., et al., The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013 Jun;20(6):687-95).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

60160124

Reads aligned (%)

91.2

Duplicates removed (%)

26.2

Number of peaks

22923 (qval < 1E-05)

hg19

Number of total reads

60160124

Reads aligned (%)

90.6

Duplicates removed (%)

27.3

Number of peaks

22662 (qval < 1E-05)