Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Unclassified
Cell type
Unclassified
NA
NA

Attributes by original data submitter

Sample

source_name
Cell culture
growth protocol
grown in RPMI supplemented with 10% FBS, penicillin and streptomycin.
experiment
ChIP-H3K4me1
antibodies
H3K4me1 (Abcam, ab8895)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Native ChIP was done as previously described (Hasson D., et al., The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013 Jun;20(6):687-95). Libraries for ChIP-seq were done as previously described (Hasson D., et al., The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013 Jun;20(6):687-95).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
108312015
Reads aligned (%)
97.9
Duplicates removed (%)
12.5
Number of peaks
44387 (qval < 1E-05)

hg19

Number of total reads
108312015
Reads aligned (%)
97.6
Duplicates removed (%)
13.2
Number of peaks
43303 (qval < 1E-05)

Base call quality data from DBCLS SRA