Antigen

Antigen Class

TFs and others

Antigen

KDM1A

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

Prostate cancer cell line LNCaP

cell type

LNCaP

transfection

no treatment

passage

14-17

antibody

LSD1 antibody (Abcam, ab17721, lot # GR79477-2)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

LNCaP and VCaP cells were harvested by scraping from the plates. Libraries were prepared according to Rubicon's instructions accompanying the ThruPLEX-FD kit (for ChIP-seq) or Illumina TruSeq RNA Sample Prep Kit v2 (for RNA-seq)

Sequencing Platform

instrument_model

Illumina HiSeq 1000

hg38

Number of total reads

10625482

Reads aligned (%)

92.4

Duplicates removed (%)

51.1

Number of peaks

1257 (qval < 1E-05)

hg19

Number of total reads

10625482

Reads aligned (%)

91.7

Duplicates removed (%)

52.7

Number of peaks

1281 (qval < 1E-05)