Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KDM1A

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
Prostate cancer cell line LNCaP
cell type
LNCaP
transfection
no treatment
passage
14-17
antibody
LSD1 antibody (Abcam, ab17721, lot # GR79477-2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
LNCaP and VCaP cells were harvested by scraping from the plates. Libraries were prepared according to Rubicon's instructions accompanying the ThruPLEX-FD kit (for ChIP-seq) or Illumina TruSeq RNA Sample Prep Kit v2 (for RNA-seq)

Sequencing Platform

instrument_model
Illumina HiSeq 1000

hg19

Number of total reads
10625482
Reads aligned (%)
43.4
Duplicates removed (%)
52.7
Number of peaks
1281 (qval < 1E-05)

hg38

Number of total reads
10625482
Reads aligned (%)
45.2
Duplicates removed (%)
51.1
Number of peaks
1257 (qval < 1E-05)

Base call quality data from DBCLS SRA