Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
Embryonic face
NA
NA

Attributes by original data submitter

Sample

source_name
facial mesenchyme
background strain
C57BL6/j
genotype/variation
wt
embryonic stage
11.5
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from drbris of nuclei and DNA-wrapped histones were Immunoprecipitated with specific antibody. Libraries were prepared with NEB Next ChIP-seq Sample Prep Master Mix Set I (Cat.No.E6240S, NEB) and NEB Next Multiplex Oligos for Illumina (Cat.No: E7335S, NEB)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
21963301
Reads aligned (%)
98.5
Duplicates removed (%)
14.2
Number of peaks
413 (qval < 1E-05)

mm9

Number of total reads
21963301
Reads aligned (%)
98.3
Duplicates removed (%)
14.3
Number of peaks
460 (qval < 1E-05)

Base call quality data from DBCLS SRA