Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Digestive tract

Cell type

Colorectal cancer organoid

NA

NA

Sample

source_name

Input DNA for REST ChIP-seq in context of organoid integrity

tissue

CRC121 organoid

chip antibody

None

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Extraction of material for sequencing was performed as in http://dx.doi.org/10.1038/nature13736 Libraries were prepared according to Roche's instructions accompanying the kapa htp library prep kit, and DNA was amplified with 12 PCR cycles.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

51561143

Reads aligned (%)

97.9

Duplicates removed (%)

11.6

Number of peaks

1058 (qval < 1E-05)

hg19

Number of total reads

51561143

Reads aligned (%)

96.9

Duplicates removed (%)

12.0

Number of peaks

514 (qval < 1E-05)