Antigen

Antigen Class

TFs and others

Antigen

REST

Cell type

Cell type Class

Digestive tract

Cell type

Colorectal cancer organoid

NA

NA

Sample

source_name

ChIP-seq of REST in context of shRNA knockdown of CDH1

tissue

CRC107 organoid

chip antibody

REST (Millipore Sigma, 07-579, lot 2843200)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Extraction of material for sequencing was performed as in http://dx.doi.org/10.1038/nature13736 Libraries were prepared according to Roche's instructions accompanying the kapa htp library prep kit, and DNA was amplified with 12 PCR cycles.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

54070528

Reads aligned (%)

95.9

Duplicates removed (%)

20.5

Number of peaks

23841 (qval < 1E-05)

hg19

Number of total reads

54070528

Reads aligned (%)

95.4

Duplicates removed (%)

20.7

Number of peaks

23465 (qval < 1E-05)